Journal: Arteriosclerosis, Thrombosis, and Vascular Biology

Article Title: Divergent Effects of p47 phox Phosphorylation at S303-4 or S379 on Tumor Necrosis Factor-α Signaling via TRAF4 and MAPK in Endothelial Cells

doi: 10.1161/atvbaha.112.247775

Figure Lengend Snippet: Figure 5. Experiments using p47phox knockout (KO) coro- nary microvascular endothe- lial cell (CMEC) or COS7phox cells. A, NADPH-dependent O2 .2 production measured by lucigenin chemiluminescence. B, Extracellular-signal–regulated kinase (ERK) 1/2 phosphoryla- tion. *P0.05 for indicated values versus vehicle values in the same treatment group. C, Effect of pro- tein kinase C (PKC) inhibitor (bis- indolylmaleimide [BIM]) on tumor necrosis factor-a (TNFa)–induced p47phox phosphorylation. *P0.05 for indicated values versus vec- tor values in the same treatment group.

Article Snippet: A mouse lymph node microvascular endothelial cell line (SVEC4-10) was obtained from the American Type Culture Collection.

Techniques: Knock-Out, Phospho-proteomics

Journal: Arteriosclerosis, Thrombosis, and Vascular Biology

Article Title: Divergent Effects of p47 phox Phosphorylation at S303-4 or S379 on Tumor Necrosis Factor-α Signaling via TRAF4 and MAPK in Endothelial Cells

doi: 10.1161/atvbaha.112.247775

Figure Lengend Snippet: Figure 6. Tumor necrosis factor-a (TNFa)–induced p47phox membrane translocation and vascular cell adhesion molecule (VCAM)-1 expression in p47phox knockout (KO) coronary microvascular endothelial cell (CMEC) after gene transfection. A, p47phox immunoprecipi- tates detected for the presence of TNF receptor–associated factor 4 (TRAF4). B, Membrane fractions detected for p47phox membrane translocation and VCAM-1 expression. *P0.05 for indicated values versus the WTp47 values. C, Cell homogenates detected for the effects of U0126 on TNFa-induced VCAM-1 expression. *P0.05 for indicated values versus the vector values in the same treatment group. D, Confocal microscopy. Nuclei are labeled by propidium iodide (PI, red) and VCAM-1 is labeled by fluorescein isothiocyanate (FITC) (green).

Article Snippet: A mouse lymph node microvascular endothelial cell line (SVEC4-10) was obtained from the American Type Culture Collection.

Techniques: Membrane, Translocation Assay, Expressing, Knock-Out, Transfection, Plasmid Preparation, Confocal Microscopy, Labeling

Journal: Cell

Article Title: Serum Amyloid A Proteins Induce Pathogenic T H 17 Cells and Promote Inflammatory Disease

doi: 10.1016/j.cell.2019.11.026

Figure Lengend Snippet: KEY RESOURCE TABLE

Article Snippet: On day 5 of culture, cells were reactivated on plates precoated with 2 μg/ml of anti-CD3 and anti-CD28 (PV1, BioXCell) for an additional 48 h, before adoptive transfer.

Techniques: Flow Cytometry, Western Blot, In Vitro, Cell Differentiation, Activation Assay, In Vivo, Immunohistochemistry, Recombinant, Enzyme-linked Immunosorbent Assay, Staining, SYBR Green Assay, Ex Vivo, Isolation, Knock-In, Knock-Out, Software